Protein Molecular Weight Determination Using MALDI

  • Brand: Shrimpex Biotech Services

  • Product Code: PROT03

  • Availability: Pre-Order

  • Seller: TechnoConcept India Pvt. Ltd.

  • Delivery: Dispatch will be made in 7-10 days

Price: ₹1,320.00

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Please refer to the following steps for sending in your samples:

  1. Please note that prior payment must be made to avail this service. 
  2. Once you place the order for Protein Identification by LC/MS on labekart.com, please schedule your sample pick up date & time so that our logistics partner can pick up the sample from your place. 
  3. You MUST write your address, mobile no. and e-mail id in CAPITAL LETTERS. 
  4. Please download the following form and attach the duly filled in form alongwith your package. Your package won't be accepted without this form.
  5. Pick up will be informed prior and will be attempted twice. In case you are not availble even after second attempt, we will credit your money deducting pick up charges in your LabeKart account. 
  6. Once samples are picked up from your place, you must send us an email at customer.care@labekart.com mentioning your Order ID No. 
  • Results are sent by e-mail on the e-mail id mentioned by you in form. 
  • Results for Samples that are picked up on days before Wednesday of a week are sent by the Wednesday of the following week.
  • Samples which are NOT qualified at the facility of BGI are not run for sequencing.

Molecular weight determination can be used for verification of a formula of low molecular weight substances, and for verification of the identity of a purified protein. It is possible to determine molecular weights of biopolymers and chemical substances with high accuracy and high resolution.Mass spectrometric analysis provides the molecular weight (Mw) of your intact protein. MALDI-MS analysis works well for small and large proteins. The Mw determination is most accurate for proteins with Mw below 50 kDa.

*Disclaimer: The original products may differ from the images displayed on the screen.    

  1. Sample should be in lyophilised form
  2. MALDI Analysis is performed for samples below 50 kDa.

Liquid or solid samples

  1. Purify the protein/peptide

    1. The chromatographic protein/peptide purity should be >90%
    2. Avoid detergents and keep buffer concentration at a minimum. LC-ESI MS and MALDI MS analysis can be done on samples containing small amounts of salts, urea or detergent, but the best result are obtained with low buffer strength in volatile solvents without detergents 
    3. Minimum amount ~ 10-50 micrograms
       
  2. Put the protein/peptide into microcentrifuge tube

    1. Use the Eppendorf Safe Lock tube supplied with the kit, or similar tubes from your lab
    2. Lyophilize  the protein to a solid sample to ensure protein stability during shipment
  3. Attention

    1. Please note that MW determination of intact proteins by mass spectrometryCANNOT be conducted on samples from gels or PVDF membranes!

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Please mention your Order ID for any queries. 

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