Protein Identification by Mass Spectrometry (LC/MS)

  • Brand: Shrimpex Biotech Services

  • Product Code: PROT02

  • Availability: Pre-Order

  • Seller: TechnoConcept India Pvt. Ltd.

  • Delivery: Dispatch will be made in 7-10 days

Price: ₹13,970.00
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Protein identification can be performed on a variety of samples, including protein gel spots, protein gel bands, IP samples, etc. Proteins are analyzed either by MALDI-TOF/TOF or by nano-LC-ESI-MS/MS and then identified using peptide masses or MS/MS fragmentation spectra. We provide your protein identification results in an electronic report that also includes bioinformatics analysis results, such as GO and pathway analysis. For RMM >6000amu LC/MS is preferred.

Benefits:

  • High resolution and accuracy: With LC-MS/MS, the accurate identification results can be provided based on resolving power up to 100,000 FWHM and 1 ppm mass accuracy.
  • Ultra-fast scanning speed: UltrafleXtremeTM MALDI-TOF/TOF MS with 1 kHz smartbeam IITM laser technology can provide fast spectra collection in both TOF and TOF/ TOF modes.
  • High sensitivity: Able to detect proteins at even nanogram levels.
  • Protein identification aims to identify peptides and proteins from gel spot samples or gel band samples. It can provide necessary fundamental knowledge for further proteomics analysis.

 

Sample Requirements for Protein Identification:

  • Gel band: the amount of proteins ≥30µg, concentration ≥1µg/µl. The gel band needs to be visible with coomassie blue or silver staining.
  • Gel spot: the amount of proteins ≥50pmol. Salt content: volatile inorganic salt < 20mM, stable inorganic salt < 5mM.
  • Protein molecular weight detection:
    • Sample type: purified protein solution or powder (the purity of the sample should be > 90%)
    • Total amount of protein: ≥50ug
    • Protein concentration: ≥1ug/ul
  • If silver staining method is used, please be sure not to fix the gel with glutaraldehyde.

Sample Preparation:

1. Sample preparation: enzymolysis
2. MS analysis strategy:
2.1 gel spot samples: ultrafleXtreme MS analysis
2.2 gel band samples: LTQ-Orbitrap velos MS analysis

Please refer to the following steps for sending in your samples:

  1. Please note that prior payment must be made to avail this service. 
  2. Once you place the order for Protein Identification by LC/MS on labekart.com, please schedule your sample pick up date & time so that our logistics partner can pick up the sample from your place. 
  3. You MUST write your address, mobile no. and e-mail id in CAPITAL LETTERS. 
  4. Please download the following form and attach the duly filled in form alongwith your package. Your package won't be accepted without this form.
  5. Pick up will be informed prior and will be attempted twice. In case you are not availble even after second attempt, we will credit your money deducting pick up charges in your LabeKart account. 
  6. Once samples are picked up from your place, you must send us an email at customer.care@labekart.com mentioning your Order ID No. 
  • Results are sent by e-mail on the e-mail id mentioned by you in form. 
  • Results for Samples that are picked up on days before Wednesday of a week are sent by the Wednesday of the following week.
  • Samples which are NOT qualified at the facility of BGI are not run for sequencing.

For tech support please contact us at customer.care@labekart.com or +919999151608

Please mention your Order ID for any queries. 

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