Your shopping cart is empty!
Brand: Shrimpex Biotech Services
Product Code: GEN04
Seller: TechnoConcept India Pvt. Ltd.
Delivery: Dispatch will be made in 7-10 days
Primer walking is a technique used to sequence large DNA fragements of above 1 kb (upto 10kb). Sequencing the unknown fragment. Sequencing will be performed from each 5' and 3' ends using the appropriate vector or PCR primers. From the sequence information an new primer will be designed to sequence the next 1kb sequence. and this continues till the whole large fragment is covered.
PLEASE NOTE: Cost of sequencing: Rs. 6.60 per base. Please multiply this cost with the length of your DNA fragment.
*Disclaimer: The original products may differ from the images displayed on the screen.
Plasmid - Submit 1 µg per kb of target sequence for single stranded coverage and 2 µg per kb sequence for double stranded coverage. Submit at least 10 µg plasmid if region of interest is of unknown length.
Primers - If you have primers that can be used in the initial round of sequencing, please submit a minimum of 10 µl each at 5 µM.
Packaging - For DNA and primers, please use 1.5 ml screw cap tubes and label them with sample or primer name and concentration.
Please refer to the following steps for sending in your samples: