Primer Walking by Sanger's Method, Bidirectional, Rs. 6.6/base

  • Brand: Shrimpex Biotech Services

  • Product Code: GEN04

  • Availability: Pre-Order

  • Seller: TechnoConcept India Pvt. Ltd.

  • Delivery: Dispatch will be made in 7-10 days

Price: ₹7.26

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Primer walking is a technique used to sequence large DNA fragements of above 1 kb (upto 10kb). Sequencing the unknown fragment. Sequencing will be performed from each 5' and 3' ends using the appropriate vector or PCR primers. From the sequence information an new primer will be designed to sequence the next 1kb sequence. and this continues till the whole large fragment is covered.    

PLEASE NOTE: Cost of sequencing: Rs. 6.60 per base. Please multiply this cost with the length of your DNA fragment. 

*Disclaimer: The original products may differ from the images displayed on the screen.    

Plasmid - Submit 1 µg per kb of target sequence for single stranded coverage and 2 µg per kb sequence for double stranded coverage. Submit at least 10 µg plasmid if region of interest is of unknown length.
Primers - If you have primers that can be used in the initial round of sequencing, please submit a minimum of 10 µl each at 5 µM.
Packaging - For DNA and primers, please use 1.5 ml screw cap tubes and label them with sample or primer name and concentration.

Please refer to the following steps for sending in your samples:

  1. For processing your order on DNA sequencing, your advised to login to your account and then complete your purchase. 
  2. Please note that prior payment must be made to avail this service. 
  3. Once you place the order for DNA Sequencing on, please schedule your sample pick up date & time so that our logistics partner can pick up the sample from your place. 
  4. HOW TO PROCESS YOUR ORDER: Once you have completed your purchase, you are advised to login to your account. Please visit "My account">"My Orders">"Downloads" to be able to download the Order Form-DNA Sequencing. (The form will be available only upon successful completion of your purchase). Please fill in the order form and send it along with your package. Your order won't be accepted and/or processed without the order form. You are also required to send us the electronic copy of the document at quoting your order ID. 
  5. You MUST write your address, mobile no. and e-mail id in CAPITAL LETTERS. 
  6. Pick up will be informed prior and will be attempted twice. In case you are not availble even after second attempt, we will credit your money deducting pick up charges in your LabeKart account. 
  7. Once samples are picked up from your place, you must send us an email at mentioning your Order ID No. 

  • Results are sent by e-mail on the e-mail id mentioned by you in form. 
  • Results for Samples that are picked up on days before Wednesday of a week are sent by the Wednesday of the following week.
  • Samples which are NOT qualified at the facility of BGI are not run for sequencing. 
  • We can adjust DNA Sequencing charges in the next sequencing reactions, however, sample pick up charges and service tax paid will not be adjusted.