The RNA Paranoia!

The RNA Paranoia!

Write By: admin Published In: Taking Care Created Date: 2016-09-09 Hits: 489 Comment: 0

Of all the “desktop” molecular biology experiments that I have been exposed to, this one wins the prize for making your neurons fire at another level of crazy. The stimulus driving such hyperactivity is due to the “You-Know-Who” RNase! This little bundle of terror refuses to die down, give way or just stand in the corner for a while to let your experiments proceed at a decent pace. It finds a way right into the undesired places, extending your PhD one week at a time. 

Of all the “desktop” molecular biology experiments that I have been exposed to, this one wins the prize for making your neurons fire at another level of crazy. The stimulus driving such hyperactivity is due to the “You-Know-Who” RNase! This little bundle of terror refuses to die down, give way or just stand in the corner for a while to let your experiments proceed at a decent pace. It finds a way right into the undesired places, extending your PhD one week at a time.

When I started working to extract RNA for an internship project, I knew the theory but I failed to realize the intensity. Before I could even start reading the protocol my guide made me clean every inch of the workspace and required equipments first with 70% ethanol followed by this special solution of ‘RNase away’ after which she wouldn’t let me so much as even flip the page of the protocol notebook. I diagnosed her with OCD right away only to realize later how intense this ‘RNA paranoia’ was. All you had to do was announce that you’re handling RNA and everyone would make way for you. The reason however become apparent only hours later when our RNA gel shined with sharper bands and no smears and my friend’s gel depicted all the cleaved up fragments probably because her guide wasn’t paranoid enough!

For anyone who has worked with RNA would realize how difficult it is to keep RNase really away. With Labekart’s RNase-free microcentrifuge tubes and sterilized Cole-Parmer Nitrile blue gloves available just a click away you can deal with at least half of your problem! 

Tags: RNA RNASES

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